RefFinder
is a user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets. It integrates the currently available major computational programs (geNorm , Normfinder , BestKeeper , and the comparative Δ-Ct method ) to compare and rank the tested candidate reference genes. Based on the rankings from each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking. LIZhen ( ORCID: 0000-0002-8512-2554 ) established and maintained the RefFinder mirror site ( http://blooge.cn/RefFinder/ ). Please thanks for LIZhen (Sichuan Academy of Botanical Engineering) contributed analytical tool : RefFinder ( http://blooge.cn/RefFinder/ ) in Acknowledgements or Contributions ( just like DOI: 10.1038/s41598-022-16124-3 & 10.3389/fmicb.2023.1140515 ) .
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References
RefFinder ( DOI: 10.1007/s11103-012-9885-2 )
Genorm ( DOI: 10.1186/gb-2002-3-7-research0034 )
NormFinder ( DOI: 10.1158/0008-5472.can-04-0496 )
BestKeeper ( DOI: 10.1023/b:bile.0000019559.84305.47 )
The comparative delta-Ct method ( DOI: 10.1186/1471-2199-7-33 )
Citations RefFinder ( http://blooge.cn/RefFinder/ )
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稻秆潜蝇的生物学特性及化学防治技术研究
20210713 DOI: 10.21203/rs.3.rs-645521/v1 Research Square (preprint)
Evaluation And Validation of Reliable Reference Genes For Quantitative Real-Time PCR Analysis of The Gene Expression In Macadamia Integrifolia
20210727 DOI: 10.1101/2021.07.27.454008 bioRχiv (preprint)
Selection and evaluation of qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae)
20210819 DOI: 10.1038/s41598-021-96055-7 Scientific Reports (IF=4.38 )
Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR
20210909 DOI: 10.1089/dna.2021.0185 DNA and Cell Biology (IF=3.311 )
Evaluation of Reliable Reference Genes for In Vitro Erythrocyte Generation from Cord Blood CD34+ Cells
20210923 DOI: 10.19540/j.cnki.cjcmm.20210919.101 中国中药杂志 (中文)
艾Artemisia argyi实时荧光定量PCR内参基因筛选
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The Era of Antimicrobial Peptides: Use of Hepcidins to Prevent or Treat Bacterial Infections and Iron Disorders
20211021 DOI: 10.3389/fgene.2021.716137 Frontiers in Genetics (IF=4.599 )
Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and Response to Hormone Stimuli
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Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
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Identification And Evaluation of Reference Genes For Cynanchum Auriculatum Under Various Stress Conditions
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Selection and evaluation of RT-qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi matsumura (Hymenoptera: Trichogrammatidae)
20220401 CNKI: http://kns.cnki.net/kcms/detail/22.1100.S.20220331.1519.003.html 吉林农业大学学报 (中文)
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Accurate reflection of hepatopancreas antioxidation and detoxification in Procambarus clarkii during virus infection and drug treatment: Reference gene selection, evaluation and expression analysis
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Protective Roles of Sodium Butyrate in Lipopolysaccharide-Induced Bovine Ruminal Epithelial Cells by Activating G Protein-Coupled Receptors 41
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Screening of new reference genes for qRT-PCR normalisation in rice under heat stress
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光周期对稻螟赤眼蜂行为及与生物钟相关基因表达模式的影响
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Identification of Auxiliary Biomarkers and Description of the Immune Microenvironmental Characteristics in Duchenne Muscular Dystrophy by Bioinformatical Analysis and Experiment
20220606 DOI: 10.3389/fpls.2022.893495 Frontiers in Plant Science (IF=5.754 )
Identification of Reference Genes for Reverse Transcription-Quantitative PCR Analysis of Ginger Under Abiotic Stress and for Postharvest Biology Studies
20220610 DOI: 10.3390/v14061269 Viruses (IF=5.048 )
Selection and Validation of Reference Genes for Gene Expression Studies Using Quantitative Real-Time PCR in Prunus Necrotic Ringspot Virus-Infected Cucumis sativus
20220615 DOI: 10.21203/rs.3.rs-1759142/v1 Research Square (preprint)
Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb using quantitative real-time PCR
------------------ ↑ 2020 IF ↑ ------------------ ↓ 2021 IF ↓ ------------------
20220708 DOI: 10.13345/j.cjb.220345 生物工程学报 (中文)
钩吻看家基因筛选及生物碱合成相关酶基因的表达分析
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Identification of Reference Genes for RT-qPCR Analysis in Gleditsia microphylla under Abiotic Stress and Hormone Treatment
20220712 DOI: 10.1038/s41467-022-31802-6 Nature Communications (IF=17.694 )
Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton
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Identification of internal reference genes for porcine immature Sertoli cells under heat stress
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Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)
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Co-overexpression of self-renewal markers SALL4 and HIWI is correlated with depth of tumor invasion and metastasis in colorectal cancer
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Nutrient Starvation Exposure Induced the Down-Regulation of Stress Responsive Genes and Selected Bioactive Metabolic Pathways in Phaeodactylum tricornutum
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Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
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柑橘黄龙病菌内参基因metG筛选和应用
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Validation of Appropriate Reference Genes for qRT–PCR Normalization in Oat (Avena sativa L.) under UV-B and High-Light Stresses
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Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR
20221017 DOI: 10.7717/peerj.14221 Peer J (IF=3.061 )
Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms
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Selection and validation of reference genes for RT-qPCR-based analyses of Anastatus japonicus Ashmead (Hymenoptera: Helicopteridae)
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20221113 DOI: 10.3390/insects13111046 Insects (IF=3.139 )
Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
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Impact of TRIM5α and TRIM22 Genes Expression on the Clinical Course of Coronavirus Disease 2019
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