RefFinder
2020.10.20 - 2023.06.10

Evaluating Reference Genes Expression>>
RefFinder is a user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets. It integrates the currently available major computational programs (geNorm, Normfinder, BestKeeper, and the comparative Δ-Ct method) to compare and rank the tested candidate reference genes. Based on the rankings from each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking. LIZhen ( ORCID: 0000-0002-8512-2554 ) established and maintained the RefFinder mirror site ( http://blooge.cn/RefFinder/ ). Please thanks for LIZhen ( Sichuan Academy of Agricultural Characteristic Plants ) contributed analytical tool : RefFinder ( http://blooge.cn/RefFinder/ ) in Acknowledgements or Contributions ( just like DOI: 10.1007/s10142-023-01055-7 , 10.1038/s41598-022-16124-3 , 10.3389/fmicb.2023.1140515 , 10.3390/biomedicines11041079 ) .
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References
  1. RefFinder ( DOI: 10.1007/s11103-012-9885-2 (2012) , 10.1007/s10142-023-01055-7 (2023) )
  2. Genorm ( DOI: 10.1186/gb-2002-3-7-research0034 )
  3. NormFinder ( DOI: 10.1158/0008-5472.can-04-0496 )
  4. BestKeeper ( DOI: 10.1023/b:bile.0000019559.84305.47 )
  5. The comparative delta-Ct method ( DOI: 10.1186/1471-2199-7-33 )
Citations
    RefFinder ( http://blooge.cn/RefFinder/ )
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      稻秆潜蝇的生物学特性及化学防治技术研究
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      Evaluation And Validation of Reliable Reference Genes For Quantitative Real-Time PCR Analysis of The Gene Expression In Macadamia Integrifolia
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      Selection and evaluation of qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae)
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      Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR
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      Evaluation of Reliable Reference Genes for In Vitro Erythrocyte Generation from Cord Blood CD34+ Cells
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      ------------------ ↑ 2020 IF ↑ ------------------ ↓ 2021 IF ↓ ------------------
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      Identification of internal reference genes for porcine immature Sertoli cells under heat stress
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      Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)
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      Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
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      Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR
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      Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms
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      Selection of Reference Genes for Normalization of qRT‒PCR Analysis in the Soybean Aphid Aphis glycines Matsumura (Hemiptera: Aphididae)
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      Selection and validation of reference genes for RT-qPCR-based analyses of Anastatus japonicus Ashmead (Hymenoptera: Helicopteridae)
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      Systematic Identification and Validation of Suitable Reference Genes for the Normalization of Gene Expression in Prunella vulgaris under Different Organs and Spike Development Stages
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      Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
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      DNA methylation dynamics in a coastal foundation seagrass species under abiotic stressors
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      Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata
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      Transcriptome-Based Evaluation of Optimal Reference Genes for Quantitative Real-Time PCR in Yak Stomach throughout the Growth Cycle
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      Housekeeping gene stability in Pesudomonas aeruginosa PAO1 under the pressure of commonly used antibiotics in molecular microbiology assays
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