RefFinder
is a user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets. It integrates the currently available major computational programs (geNorm , Normfinder , BestKeeper , and the comparative Δ-Ct method ) to compare and rank the tested candidate reference genes. Based on the rankings from each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking. LIZhen ( ORCID: 0000-0002-8512-2554 ) established and maintained the RefFinder mirror site ( http://blooge.cn/RefFinder/ ). Please thanks for LIZhen ( Sichuan Academy of Agricultural Characteristic Plants ) contributed analytical tool : RefFinder ( http://blooge.cn/RefFinder/ ) in Acknowledgements or Contributions ( just like DOI: 10.1007/s10142-023-01055-7 , 10.1038/s41598-022-16124-3 , 10.3389/fmicb.2023.1140515 , 10.3390/biomedicines11041079 ) .
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References
RefFinder ( DOI: 10.1007/s11103-012-9885-2 (2012) , 10.1007/s10142-023-01055-7 (2023) )
Genorm ( DOI: 10.1186/gb-2002-3-7-research0034 )
NormFinder ( DOI: 10.1158/0008-5472.can-04-0496 )
BestKeeper ( DOI: 10.1023/b:bile.0000019559.84305.47 )
The comparative delta-Ct method ( DOI: 10.1186/1471-2199-7-33 )
Citations RefFinder ( http://blooge.cn/RefFinder/ )
20210601 DOI: 10.27136/d.cnki.ghunu.2021.000130 硕士论文 (中文)
稻秆潜蝇的生物学特性及化学防治技术研究
20210713 DOI: 10.21203/rs.3.rs-645521/v1 Research Square (preprint)
Evaluation And Validation of Reliable Reference Genes For Quantitative Real-Time PCR Analysis of The Gene Expression In Macadamia Integrifolia
20210727 DOI: 10.1101/2021.07.27.454008 bioRχiv (preprint)
Selection and evaluation of qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae)
20210819 DOI: 10.1038/s41598-021-96055-7 Scientific Reports (IF=4.38 )
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20210909 DOI: 10.1089/dna.2021.0185 DNA and Cell Biology (IF=3.311 )
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20210923 DOI: 10.19540/j.cnki.cjcmm.20210919.101 中国中药杂志 (中文)
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The Era of Antimicrobial Peptides: Use of Hepcidins to Prevent or Treat Bacterial Infections and Iron Disorders
20211021 DOI: 10.3389/fgene.2021.716137 Frontiers in Genetics (IF=4.599 )
Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and Response to Hormone Stimuli
20211029 DOI: 10.1080/1828051X.2021.1992304 Italian Journal of Animal Science (IF=2.217 )
Growth performance, meat quality and lipid metabolism in finishing lambs fed diets containing rumen-unprotected and rumen-protected betaine
20211115 DOI: 10.16409/j.cnki.2095-039x.2021.11.004 中国生物防治学报 (中文)
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20211118 DOI: 10.3390/ani11113295 Animals (IF=2.752 )
Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
20211230 DOI: 10.13422/j.cnki.syfjx.20220313 中国实验方剂学杂志 (中文)
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20220110 DOI: 10.21203/rs.3.rs-1196107/v1 Research Square (preprint)
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20220112 DOI: 10.3389/fmicb.2021.827241 Frontiers in Microbiology (IF=5.64 )
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20220201 DOI: 10.1016/j.aspen.2022.101883 Journal of Asia-Pacific Entomology (IF=1.303 )
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20220506 DOI: 10.3389/fnut.2022.842634 Frontiers in Nutrition (IF=6.576 )
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20220512 DOI: 10.27163/d.cnki.gjlnu.2022.000018 硕士论文 (中文)
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光周期对稻螟赤眼蜂行为及与生物钟相关基因表达模式的影响
20220520 DOI: 10.27408/d.cnki.gxmzc.2022.000544 硕士论文 (中文)
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20220520 DOI: 10.27345/d.cnki.gsnyu.2022.000030 博士论文 (中文)
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20220531 DOI: 10.1016/j.ygeno.2022.110393 Genomics (IF=5.736 )
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20220615 DOI: 10.21203/rs.3.rs-1759142/v1 Research Square (preprint)
Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb using quantitative real-time PCR
------------------ ↑ 2020 IF ↑ ------------------ ↓ 2021 IF ↓ ------------------
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20220712 DOI: 10.1038/s41467-022-31802-6 Nature Communications (IF=17.694 )
Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton
20220723 DOI: 10.1111/rda.14211 Reproduction in Domestic Animals (IF=1.858 )
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20220728 DOI: 10.1038/s41598-022-16124-3 Scientific Reports (IF=4.996 )
Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)
20220813 DOI: 10.1186/s43042-022-00333-6 Egyptian Journal of Medical Human Genetics
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20220815 DOI: 10.3390/stresses2030022 Stresses
Nutrient Starvation Exposure Induced the Down-Regulation of Stress Responsive Genes and Selected Bioactive Metabolic Pathways in Phaeodactylum tricornutum
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柑橘黄龙病菌内参基因metG筛选和应用
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Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR
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Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms
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20221128 DOI: 10.27409/d.cnki.gxbnu.2022.002265 博士论文 (中文)
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RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes
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