2020.10.20 - 2023.03.29

Evaluating Reference Genes Expression>>
RefFinder is a user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets. It integrates the currently available major computational programs (geNorm, Normfinder, BestKeeper, and the comparative Δ-Ct method) to compare and rank the tested candidate reference genes. Based on the rankings from each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking. LIZhen ( ORCID: 0000-0002-8512-2554 ) established and maintained the RefFinder mirror site ( ). Please thanks for LIZhen (Sichuan Academy of Botanical Engineering) contributed analytical tool : RefFinder ( ) in Acknowledgements or Contributions ( just like DOI: 10.1038/s41598-022-16124-3 & 10.3389/fmicb.2023.1140515 ) .
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  1. RefFinder ( DOI: 10.1007/s11103-012-9885-2 )
  2. Genorm ( DOI: 10.1186/gb-2002-3-7-research0034 )
  3. NormFinder ( DOI: 10.1158/0008-5472.can-04-0496 )
  4. BestKeeper ( DOI: 10.1023/b:bile.0000019559.84305.47 )
  5. The comparative delta-Ct method ( DOI: 10.1186/1471-2199-7-33 )
    RefFinder ( )
    1. 20210601 DOI: 10.27136/d.cnki.ghunu.2021.000130 硕士论文 (中文)
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      Selection and evaluation of qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae)
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      Assessment of reference genes at six different developmental stages of Schistosoma mansoni for quantitative RT-PCR
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      Evaluation of Reliable Reference Genes for In Vitro Erythrocyte Generation from Cord Blood CD34+ Cells
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      Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and Response to Hormone Stimuli
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    15. 20220201 DOI: 10.1016/j.aspen.2022.101883 Journal of Asia-Pacific Entomology (IF=1.303
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    16. 20220401 CNKI: 吉林农业大学学报 (中文)
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    19. 20220501 DOI: 10.27163/d.cnki.gjlnu.2022.000018 硕士论文 (中文)
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    20. 20220506 DOI: 10.3389/fnut.2022.842634 Frontiers in Nutrition (IF=6.576
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    21. 20220515 DOI: 10.1080/01140671.2022.2072903 New Zealand Journal of Crop and Horticultural Science (IF=1.154
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    22. 20220518 DOI: 10.26981/d.cnki.gjhsc.2022.000735 硕士论文 (中文)
    23. 20220520 DOI: 10.27408/d.cnki.gxmzc.2022.000544 硕士论文 (中文)
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    28. 20220615 DOI: 10.21203/ Research Square (preprint)
      Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb using quantitative real-time PCR
      ------------------ ↑ 2020 IF ↑ ------------------ ↓ 2021 IF ↓ ------------------
    29. 20220708 DOI: 10.13345/j.cjb.220345 生物工程学报 (中文)
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    31. 20220712 DOI: 10.1038/s41467-022-31802-6 Nature Communications (IF=17.694
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    32. 20220723 DOI: 10.1111/rda.14211 Reproduction in Domestic Animals (IF=1.858
      Identification of internal reference genes for porcine immature Sertoli cells under heat stress
    33. 20220728 DOI: 10.1038/s41598-022-16124-3 Scientific Reports (IF=4.996
      Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)
    34. 20220813 DOI: 10.1186/s43042-022-00333-6 Egyptian Journal of Medical Human Genetics (IF=0
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      Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
    37. 20220830 CN114958875A 中国专利
    38. 20220923 DOI: 10.3390/ijms231911187 International Journal of Molecular Sciences (IF=6.208
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      Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR
    40. 20221017 DOI: 10.7717/peerj.14221 Peer J (IF=3.061
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    42. 20221019 DOI: 10.3389/fphys.2022.1046204 Frontiers in Physiology (IF=4.755
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      Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
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      Evaluation of Reference Genes Suitable for Gene Expression during Root Enlargement in Cherry Radish Based on Transcriptomic Data
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      Impact of TRIM5α and TRIM22 Genes Expression on the Clinical Course of Coronavirus Disease 2019
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      Transcriptome-Based Evaluation of Optimal Reference Genes for Quantitative Real-Time PCR in Yak Stomach throughout the Growth Cycle
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    61. 20230317 DOI: 10.1038/s41598-023-31503-0 Scientific Reports (IF=4.996
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    62. 20230321 DOI: 10.3969/j.issn.1674-7968.2023.04.019 农业生物技术学报 (中文)