RefFinder
2020.10.20 - 2023.06.10

Evaluating Reference Genes Expression>>
RefFinder is a user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets. It integrates the currently available major computational programs (geNorm, Normfinder, BestKeeper, and the comparative Δ-Ct method) to compare and rank the tested candidate reference genes. Based on the rankings from each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking. LIZhen ( ORCID: 0000-0002-8512-2554 ) established and maintained the RefFinder mirror site ( http://blooge.cn/RefFinder/ ). Please thanks for LIZhen ( Sichuan Academy of Agricultural Characteristic Plants ) contributed analytical tool : RefFinder ( http://blooge.cn/RefFinder/ ) in Acknowledgements or Contributions ( just like DOI: 10.1007/s10142-023-01055-7 , 10.1038/s41598-022-16124-3 , 10.3389/fmicb.2023.1140515 , 10.3390/biomedicines11041079 ) .
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References
  1. RefFinder ( DOI: 10.1007/s11103-012-9885-2 (2012) , 10.1007/s10142-023-01055-7 (2023) )
  2. Genorm ( DOI: 10.1186/gb-2002-3-7-research0034 )
  3. NormFinder ( DOI: 10.1158/0008-5472.can-04-0496 )
  4. BestKeeper ( DOI: 10.1023/b:bile.0000019559.84305.47 )
  5. The comparative delta-Ct method ( DOI: 10.1186/1471-2199-7-33 )
Citations
    RefFinder ( http://blooge.cn/RefFinder/ )
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      ------------------ ↑ 2020 IF ↑ ------------------ ↓ 2021 IF ↓ ------------------
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      Identification of internal reference genes for porcine immature Sertoli cells under heat stress
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      Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)
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      Co-overexpression of self-renewal markers SALL4 and HIWI is correlated with depth of tumor invasion and metastasis in colorectal cancer
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      Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
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      柑橘黄龙病菌内参基因metG筛选和应用
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      Validation of Appropriate Reference Genes for qRT–PCR Normalization in Oat (Avena sativa L.) under UV-B and High-Light Stresses
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      Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms
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      Selection of Reference Genes for Normalization of qRT‒PCR Analysis in the Soybean Aphid Aphis glycines Matsumura (Hemiptera: Aphididae)
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      Selection and validation of reference genes for RT-qPCR-based analyses of Anastatus japonicus Ashmead (Hymenoptera: Helicopteridae)
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      Systematic Identification and Validation of Suitable Reference Genes for the Normalization of Gene Expression in Prunella vulgaris under Different Organs and Spike Development Stages
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      Evaluation of Reference Genes Suitable for Gene Expression during Root Enlargement in Cherry Radish Based on Transcriptomic Data
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      Selection and Validation of miRNA Reference Genes by Quantitative Real-Time PCR Analysis in Paeonia suffruticosa
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      Identification of reference genes for RT-qPCR analysis across kiwifruit species under iron deficiency conditions
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      Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata
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      Transcriptome-Based Evaluation of Optimal Reference Genes for Quantitative Real-Time PCR in Yak Stomach throughout the Growth Cycle
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      Identification and Validation of Reference Genes for Expression Analysis Using RT-qPCR in Leptocybe invasa Fisher and La Salle (Hymenoptera: Eulophidae)
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