RefFinder
is a user-friendly web-based comprehensive tool developed for evaluating and screening reference genes from extensive experimental datasets. It integrates the currently available major computational programs (geNorm , Normfinder , BestKeeper , and the comparative Δ-Ct method ) to compare and rank the tested candidate reference genes. Based on the rankings from each program, It assigns an appropriate weight to an individual gene and calculated the geometric mean of their weights for the overall final ranking. LIZhen ( ORCID: 0000-0002-8512-2554 ) established and maintained the RefFinder mirror site ( http://blooge.cn/RefFinder/ ). Please thanks for LIZhen ( Sichuan Academy of Agricultural Characteristic Plants ) contributed analytical tool : RefFinder ( http://blooge.cn/RefFinder/ ) in Acknowledgements or Contributions ( just like DOI: 10.1007/s10142-023-01055-7 , 10.1038/s41598-022-16124-3 , 10.3389/fmicb.2023.1140515 , 10.3390/biomedicines11041079 , 10.1016/j.tox.2023.153678 ) .
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References
RefFinder ( DOI: 10.1007/s11103-012-9885-2 (2012) , 10.1007/s10142-023-01055-7 (2023) )
Added at the request of the RefFinder author Baohong Zhang
"If you employed RefFinder for your study, please cite the following papers: Xie, F., Wang, J. & Zhang, B. RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes. Funct Integr Genomics 23, 125 (2023). DOI: 10.1007/s10142-023-01055-7 and Xie, F., Xiao, P., Chen, D. et al. miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol Biol 80, 75–84 (2012). DOI: 10.1007/s11103-012-9885-2"
Please respect copyright
Genorm ( DOI: 10.1186/gb-2002-3-7-research0034 )
NormFinder ( DOI: 10.1158/0008-5472.can-04-0496 )
BestKeeper ( DOI: 10.1023/b:bile.0000019559.84305.47 )
The comparative delta-Ct method ( DOI: 10.1186/1471-2199-7-33 )
Citations RefFinder ( http://blooge.cn/RefFinder/ )
20210601 DOI: 10.27136/d.cnki.ghunu.2021.000130 硕士论文 (中文)
稻秆潜蝇的生物学特性及化学防治技术研究
20210713 DOI: 10.21203/rs.3.rs-645521/v1 Research Square (preprint)
Evaluation And Validation of Reliable Reference Genes For Quantitative Real-Time PCR Analysis of The Gene Expression In Macadamia Integrifolia
20210727 DOI: 10.1101/2021.07.27.454008 bioRχiv (preprint)
Selection and evaluation of qPCR reference genes for expression analysis in the tiny egg parasitoid wasp, Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae)
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Evaluation of Reliable Reference Genes for In Vitro Erythrocyte Generation from Cord Blood CD34+ Cells
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The Era of Antimicrobial Peptides: Use of Hepcidins to Prevent or Treat Bacterial Infections and Iron Disorders
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Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and Response to Hormone Stimuli
20211029 DOI: 10.1080/1828051X.2021.1992304 Italian Journal of Animal Science (IF=2.217 )
Growth performance, meat quality and lipid metabolism in finishing lambs fed diets containing rumen-unprotected and rumen-protected betaine
20211115 DOI: 10.16409/j.cnki.2095-039x.2021.11.004 中国生物防治学报 (中文)
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20211118 DOI: 10.3390/ani11113295 Animals (IF=2.752 )
Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
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20220110 DOI: 10.21203/rs.3.rs-1196107/v1 Research Square (preprint)
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Screening of new reference genes for qRT-PCR normalisation in rice under heat stress
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光周期对稻螟赤眼蜂行为及与生物钟相关基因表达模式的影响
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Transcriptome analysis revealed hub genes for muscle growth in Indian major carp, Catla catla (Hamilton, 1822)
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Identification of Reference Genes for Reverse Transcription-Quantitative PCR Analysis of Ginger Under Abiotic Stress and for Postharvest Biology Studies
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Selection and Validation of Reference Genes for Gene Expression Studies Using Quantitative Real-Time PCR in Prunus Necrotic Ringspot Virus-Infected Cucumis sativus
20220615 DOI: 10.21203/rs.3.rs-1759142/v1 Research Square (preprint)
Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb using quantitative real-time PCR
------------------ ↑ IF2020 ↑ ------------------ ↓ IF2021 ↓ ------------------
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钩吻看家基因筛选及生物碱合成相关酶基因的表达分析
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Identification of Reference Genes for RT-qPCR Analysis in Gleditsia microphylla under Abiotic Stress and Hormone Treatment
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Trypsin is a coordinate regulator of N and P nutrients in marine phytoplankton
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Identification of internal reference genes for porcine immature Sertoli cells under heat stress
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Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.)
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Co-overexpression of self-renewal markers SALL4 and HIWI is correlated with depth of tumor invasion and metastasis in colorectal cancer
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Nutrient Starvation Exposure Induced the Down-Regulation of Stress Responsive Genes and Selected Bioactive Metabolic Pathways in Phaeodactylum tricornutum
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Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)
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柑橘黄龙病菌内参基因metG筛选和应用
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Validation of Appropriate Reference Genes for qRT–PCR Normalization in Oat (Avena sativa L.) under UV-B and High-Light Stresses
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Validation of reference genes for gene expression analysis in fruit development of Vaccinium bracteatum Thunb. using quantitative real-time PCR
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Comparison of reference gene expression stability in mouse skeletal muscle via five algorithms
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Selection of Reference Genes for Normalization of qRT‒PCR Analysis in the Soybean Aphid Aphis glycines Matsumura (Hemiptera: Aphididae)
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Evaluation of housekeeping genes for normalizing RT-qPCR and analysis of the expression patterns of WRKY1 transcription factor and rhynchophylline biosynthetic-related genes in Uncaria rhynchophylla
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Systematic Identification and Validation of Suitable Reference Genes for the Normalization of Gene Expression in Prunella vulgaris under Different Organs and Spike Development Stages
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circSMARCA5 Is an Upstream Regulator of the Expression of miR-126-3p, miR-515-5p, and Their mRNA Targets, Insulin-like Growth Factor Binding Protein 2 (IGFBP2) and NRAS Proto-Oncogene, GTPase (NRAS) in Glioblastoma
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Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
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钩藤管家基因筛选及生物碱合成相关基因的表达分析
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Impact of TRIM5α and TRIM22 Genes Expression on the Clinical Course of Coronavirus Disease 2019
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DNA methylation dynamics in a coastal foundation seagrass species under abiotic stressors
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Selection and Validation of miRNA Reference Genes by Quantitative Real-Time PCR Analysis in Paeonia suffruticosa
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Identification of reference genes for RT-qPCR analysis across kiwifruit species under iron deficiency conditions
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circRNA432 enhances the coelomocyte phagocytosis via regulating the miR-2008-ELMO1 axis in Vibrio splendidus-challenged Apostichopus japonicus
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基于全基因组鉴定华石斛优良内参基因
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Stepwise Optimization of the RT-qPCR Protocol and the Evaluation of Housekeeping Genes in Pears (Pyrus bretschneideri) under Various Hormone Treatments and Stresses
20230224 DOI: 10.3390/genes14030573 Genes (IF=4.141 )
Evaluation of Candidate Reference Genes for Gene Expression Analysis in Wild Lamiophlomis rotata
20230303 DOI: 10.3390/ani13050925 Animals (IF=3.231 )
Transcriptome-Based Evaluation of Optimal Reference Genes for Quantitative Real-Time PCR in Yak Stomach throughout the Growth Cycle
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Housekeeping gene stability in Pesudomonas aeruginosa PAO1 under the pressure of commonly used antibiotics in molecular microbiology assays
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Hiat1 as a new transporter involved in ammonia regulation
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Quantitative evaluation of endogenous reference genes for ddPCR under salt stress using a moderate halophile
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Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris)
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镉胁迫下构树qRT-PCR内参基因筛选及验证
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A Strategy for the Selection of RT-qPCR Reference Genes Based on Publicly Available Transcriptomic Datasets
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Callose synthase and xyloglucan endotransglucosylase gene expression over time in Citrus × clementina and Citrus × sinensis infected with citrus tristeza virus
20230415 DOI: 10.1007/s10142-023-01055-7 Functional & Integrative Genomics (IF=3.674 )
RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes
20230418 DOI: 10.21203/rs.3.rs-2750081/v1 Research Square (preprint)
Immune Response to Zinc Oxide Inhalation in Metal Fume Fever - Is the IL-17f the missing link?
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Stable reference gene selection for Ophiocordyceps sinensis gene expression studies under different developmental stages and light-induced conditions
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RNAi assays in the striped flea beetle (Phyllotreta striolata) suggest Psγ-COPI and PsArf1COPI as potential molecular targets for pest control
20230425 DOI: 10.1371/journal.pone.0284764 PLOS ONE (IF=3.752 )
Identification of reference genes for the normalization of retinal mRNA expression by RT-qPCR in oxygen induced retinopathy, anemia, and erythropoietin administration
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Transcriptomics and physiological analyses unveil the distinct mechanisms of ATP and glucose-6-phosphate utilization in Phaeodactylum tricornutum
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Identification and Validation of Reference Genes for Expression Analysis Using RT-qPCR in Leptocybe invasa Fisher and La Salle (Hymenoptera: Eulophidae)
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假俭草非生物胁迫下的内参基因及其引物和应用
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PFOA exposure induces aberrant glucose and lipid metabolism in the rat liver through the AMPK/mTOR pathway
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Antidiabetic and Hypolipidemic Properties of Newly Isolated Wild Lacticaseibacillus paracasei Strains in Mature Adipocytes
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Selection and Validation of Optimal RT-qPCR Reference Genes for the Normalization of Gene Expression under Different Experimental Conditions in Lindera megaphylla
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Tumor Necrosis Factor-α Mediates Inflammation-induced Early-Stage Left Ventricular Systolic Dysfunction
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Maternal diet and gut microbiome composition modulate early-life immune development
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Selection and verification of reliable internal reference genes in stem development of herbaceous peony (Paeonia lactiflora Pall.)
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Selection of Reliable Reference Genes for Gene Expression Normalization in Sagittaria trifolia
------------------ ↑ IF2021 ↑ ------------------ ↓ IF2022 ↓ ------------------
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Data Normalization of Urine miRNA Profiling from Head and Neck Cancer Patients Treated with Cisplatin
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Identification of reference genes provides insights into the determinants of self-incompatibility in Camellia oleifera
20230712 DOI: 10.3390/plants12142629 Plants (IF=4.5 )
Screening of Reference miRNA of Different Early- and Late-Flowering Tree Peony Varieties
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川赤芍实时荧光定量PCR内参基因的筛选与验证
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Regeneration of the cerebral cortex by direct chemical reprogramming of macrophages into neuronal cells in acute ischemic stroke
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Loss of Binding Capabilities in an Ecologically Important Odorant Receptor of the Fall Armyworm, Spodoptera frugiperda, by a Single Point Mutation
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Genome-wide analysis of Panonychus citri microRNAs with a focus on potential insecticidal activity of 4 microRNAs to eggs and nymphs
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Validation of reference gene stability for miRNA quantification by reverse transcription quantitative PCR in the peripheral blood of patients with COVID-19 critical illness
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Screening of Reference Genes under Biotic Stress and Hormone Treatment of Mung Bean (Vigna radiata) by Quantitative Real-Time PCR
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栝楼性别分化期花芽及不同器官内参基因的筛选
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Molecular characterization and function of hif1a and fih1 in response to acute thermal stress in American shad (Alosa sapidissima)
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Selection of Reference Genes in Evodia rutaecarpa var. officinalis and Expression Patterns of Genes Involved in Its Limonin Biosynthesis
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The Genome-Wide Identification of Stable Internal Reference Genes Related to Delayed Spoilage for Accurate qRT-PCR Normalization in Ethephon-Treated Pueraria thomsonii Benth
20230926 DOI: 10.3390/genes14101874 Genes (IF=3.5 )
Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Centipedegrass under Different Abiotic Stress
20231012 DOI: 10.3390/genes14101932 Genes (IF=3.5 )
Effect of Supplementation with Curcuma longa and Rosmarinus officinalis Extract Mixture on Acute Phase Protein, Cathelicidin, Defensin and Cytolytic Protein Gene Expression in the Livers of Young Castrated Polish White Improved Bucks
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LRP10, PGK1 and RPLP0: Best Reference Genes in Periprostatic Adipose Tissue under Obesity and Prostate Cancer Conditions
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Selection and Validation of Reference Genes for Reverse-Transcription Quantitative PCR Analysis in Sclerotium rolfsii
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Stress-Protective Role of Dietary α-Tocopherol Supplementation in Longfin Yellowtail (Seriola rivoliana) Juveniles
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不同光照条件下万圣节法师内参基因、引物及筛选方法
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非生物胁迫下耳叶牛皮消荧光定量PCR内参基因的筛选
20231102 DOI: 10.3390/plants12213751 Plants (IF=4.5 )
Identification of Accurate Reference Genes for qRT-PCR Analysis of Gene Expression in Eremochloa ophiuroides under Multiple Stresses of Phosphorus Deficiency and/or Aluminum Toxicity
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